竹节参皂苷IVa对七氟醚麻醉后老年大鼠神经元凋亡及认知功能障碍的改善作用OA

Objective To explore the effect and its possible mechanism of Chikusetsu Saponin IVa(ChIV)on neuronal apoptosis and cognitive dysfunction in elderly rats after Sevoflurane(SEV)anesthesia.Methods Sixty elderly SD rats were randomly divided into control group,SEV group,SEV+ChIV group and SEV+ChIV+EX527(SIRT1 inhibitor)group according to the random table,with 15 rats in each group.In control group,the rats were placed in an anesthesia chamber connected to a monitoring device and inhaled 20%O2 only.The rats in SEV group inhaled 4%SEV.The rats in SEV+ChIV group and SEV+ChIV+EX527 group were intraperitoneally injected with the corresponding drugs before SEV anesthesia.Morris water maze test was used to detect the escape latency,the number of crossing the platform and the dwell time in the target quadrant.New object recognition(NOR)test was used to evaluate the discrimi-nation index of short-term and long-term memory.Y maze test was used to detect the percentage of spontaneous alternation behavior.The contents of IL-6,IL-1β and TNF-α in hippocampus were detected by ELISA.HE staining was used to observe the pathological structural damage of hippocampus.TUNEL staining was used to detect the apoptosis of hippocampal neurons.The level of ROS in hip-pocampus was detected by kit.Western blot was used to detect the expressions of apoptosis(Bax,Bcl-2 and cleaved Caspase-3)and SIRT1/BDNF pathway(SIRT1 and BDNF)related proteins.Results Compared with control group,the escape latency of rats was prolonged in SEV group,the number of crossing the platform,the target quadrant residence time,the short-term and long-term memory discrimination indexes,and the percentage of spontaneous alternation behavior decreased(P<0.05),the levels of IL-6,IL-1β and TNF-α in hippocampus increased(P<0.05),the neurons were disarranged,indistinct in outline,loosely structured,and showed distortion and shrinkage,and ROS level,the apoptosis rate and the expressions of cleaved Caspase-3 and Bax increased(P<0.05),and the expressions of Bcl-2,SIRT1 and BDNF decreased(P<0.05).Compared with SEV group,the escape latency of rats in SEV+ChIV group was shortened(P<0.05),the number of crossing the platform,the target quadrant residence time,the short-term and long-term memory discrimination indexes,and the percentage of spontaneous alternation behavior increased(P<0.05),the levels of IL-6,IL-1β and TNF-α in hippocampus decreased(P<0.05),the morphological structure of hippocampus was significantly improved,ROS level,the neuronal apoptosis rate and the expressions of cleaved Caspase-3 and Bax decreased(P<0.05),and the expressions of Bcl-2,SIRT1 and BDNF increased(P<0.05).Compared with SEV+ChIV group,the escape latency of rats was prolonged in SEV+ChIV+EX527 group(P<0.05),the number of crossing the platform,the target quadrant residence time,the short-term and long-term memory discrimination indexes,and the percentage of spontaneous alternation behavior decreased(P<0.05),and the levels of IL-6,IL-1β and TNF-α in hippocampus increased(P<0.05),the hippocampal structure damage was more severe,ROS level,the neuronal apoptosis rate and the expressions of cleaved Caspase-3 and Bax increased(P<0.05),and the expressions of Bcl-2,SIRT1 and BDNF decreased(P<0.05).Conclusion ChIV may improve neuronal apoptosis,inflammation and cognitive dysfunction in rats after SEV anesthesia by activating the SIRT1/BDNF pathway.