基于LC-MS结合网络药理学、分子对接及体内验证探究"桂南"翅荚决明对鸡抗炎作用机制OA

This study aimed to investigate the regulatory mechanism of the leguminous forage Senna alata'Guinan'on the inflammatory responses in chickens.The main active ingredients were identified using liquid chromatography-mass spectrometry(LC-MS)technology,and potential targets and signaling pathways were predicted using network pharmacology.Molecular docking and in vivo experiment were used for validation.The results showed that LC-MS analysis identified nine major active components,including galangin,6-hydroxyluteolin,morin,fisetin,and kaempferol.Database screening predicted 179 potential targets,which were intersected with 2 466 chicken inflammation-related targets,yielding 101 common targets.Protein-protein interaction(PPI)network further screened 19 core targets,including protein kinase B serine/threonine kinase 1(AKT1),epidermal growth factor receptor(EGFR),and B-cell lymphoma/leukemia-2 gene(BCL2).Gene Ontology(GO)functional enrichment analysis indicated that these targets were primarily involved in biological processes such as protein phosphorylation and apoptosis.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis revealed significant enrichment in anti-inflammatory-related signaling pathways,including the Toll-like receptor signaling pathway and apoptosis.Molecular docking results demonstrated strong binding affinity between the aforementioned active components and the core targets.In vivo experiment indicated that feeding a diet containing 20%Senna alata'Guinan'reduced serum levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)in chickens.The study shows that clarifing the core active components,targets,and mechanisms by which Senna alata'Guinan'regulates inflammation in chickens,providing a reference for its development and application as an anti-inflammatory forage resource in chicken farming.