发酵饲料对斑点叉尾鮰幼鱼生长、摄食、消化及肌肉品质的影响OA

The experiment aimed to investigate the effects of fermented feed on the growth performance,feeding,digestive enzyme activity,and muscle quality of juvenile Ictalurus punctatus.A total of 216 000 healthy Ictalurus punctatus with an initial weight of(100.79±10.23)g were selected and randomly divided into three groups,with six replicates per group and 12 000 Ictalurus punctatus per replicate.The control group(group T0)was fed with basic feed throughout the entire breeding process,the interval feeding group(group T1)was fed with fermented feed every five days to replace 20%of the basic feed,and the continuous feeding group(group T2)was fed with fermented feed to replace 20%of the basic feed throughout the breeding process.The pre-test period lasted for 14 days,and the formal trial period lasted for 180 days.The results showed that compared with the group T0,the final weight(FBW),weight gain rate(WGR),specific growth rate(SGR),and condition factor(CF)in group T1 were significantly increased(P<0.05),while the feed coefficient(FCR),visceral index(VSI),hepatosomatic index(HSI),and intestosomatic index(ISI)were significantly decreased(P<0.05).Compared with the group T0,the mRNA expression levels of agouti-related protein(agrp),neuropeptide Y(npy),and ghrelin in the groups T1 and T2 were significantly upregulated(P<0.05),while the mRNA expression levels of thyrotropin releasing hormone(trh),leptin receptor(leptin),proopiomelanocortin(pomc),leptin,cocaine-amphetamine regulated transcript(cart),and cholecystokinin(cck)were significantly downregulated(P<0.05).The activities of α-amylase(AMS),trypsin(TRY),lipase(LPS),and sodium potassium adenosine triphosphatase(Na+-K+-ATPase)in the intestinal and pancreatic segments of groups T1 and T2 were significantly increased(P<0.05),while there were no significant differences in nutritional composition of the whole fish and muscle quality(P>0.05).The study shows that fermented feed can improve the growth performance,promote expression of appetite genes,and enhance digestive enzyme activity of juvenile Ictalurus punctatus.In actual production,it is recommended to adopt the feeding method of using fermented feed every five days to replace 20%of the basic feed.