不同养殖模式对仿刺参肠道酶活性、组织结构及菌群组成的影响OA

This experiment aimed to investigate the effects of different aquaculture modes on the intestinal enzyme activities,tissue structure,and microbiota composition of Apostichopus japonicus.A total of 360 healthy Apostichopus japonicus with intact appearance,normal body color,and consistent size were randomly divided into two groups,with three replicates per group and 60 Apostichopus japonicus per replicate.The hanging cage group was cultured in hanging cages and fed with kelp and fish surimi,while the net cage group was cultured in net cages and fed with formulated feed.The pre-test period was seven days,and the formal test period was 60 days.The results showed that the activities of acid phosphatase(ACP),catalase(CAT),alkaline phosphatase(AKP),and superoxide dismutase(SOD)in the net cage group were significantly higher than those in the hanging cage group(P<0.05).The activities of amylase(AMS)and lipase(LPS)in the hanging cage group were significantly higher than those in the net cage group(P<0.05).The intestinal villus width(VW),villus height(VH),and muscularis thickness(MT)in the hanging cage group were significantly higher than those in the net cage group(P<0.05).Miseq high-throughput sequencing analysis based on 16S rRNA revealed that the intestinal microbiota of the hanging cage group encompassed 16 phylum,112 genus,and 89 species,while that of the net cage group encompassed 18 phylum,108 genus,and 65 species.There were significant differences in microbial community structure between the two aquaculture modes,the dominant bacterial genera in the hanging cage group were Vibrio,Photobacterium,Aliivibrio,Formosa,and Pseudoalteromonas.The dominant bacterial genera in the net cage group were Vibrio,Photobacterium,Shewanella,Aliivibrio,and Pseudoalteromonas.The study shows that Apostichopus japonicus cultured in net cages exhibited higher antioxidant capacity,along with significantly higher richness and diversity of intestinal microbiota compared to those cultured in hanging cages,indicating a better culture effect.