不同性别藏羚肠道菌群分析OA

Gut microbiota plays a crucial role in host physiological homeostasis and is influenced by many factors.We employed high-throughput sequencing of the 16S rDNA and bioinformatic analysis to investigate the composition and abundance of the gut microbiota of male and female Tibetan antelopes(Pantholops hodgsonii).The results showed that 6 896 OTUs were obtained from female,5 609 OTUs from male,and 2 650 OTUs were shared between male and fe-male.Female Tibetan antelopes had a higher bacterial richness than males(P＜0.05).Firmicutes and Bacteroidota were the predominant phyla in the microbial community.At the genus level,the relative abundance of 12 genera,includ-ing Aeromonas,Pseudomonas,and Methylobacterium,in the intestinal tract of male Tibetan antelope was significantly higher than that of female(P＜0.05).The relative abundance of 16 bacteria genera,including Ruminococcaceae_UCG,Akkermansia,and Peptococcus,in the intestinal tract of female Tibetan antelope was significantly higher than that of male(P＜0.05).Principal coordinate analysis revealed a significant difference in the structure of the intestinal flora be-tween the two groups,and the reproducibility of female samples was better than that of male samples.Functional predic-tion analyses revealed that the gut microbiota of Tibetan antelopes was mainly involved in carbohydrate metabolism,ami-no acid metabolism,metabolism of cofactors and vitamins,metabolism of terpenoids and polyketides,and no significant differences in metabolic pathways were found between females and males.This study explored associations between sex of the host and their gut microbiota,which may provide a reference for further understanding of the adaptive evolution-ary mechanism of Tibetan antelopes to extreme high-altitude environments.