塔里木马鹿PRDX1基因表达载体的构建及其生物信息学分析OA

To investigate the structural and functional characteristics of Peroxiredoxin 1(PRDX1),which may play an antioxidant role in the adaptation of the Tarim red deer(Cervus elaphus yarkandensis)to arid environments,this study amplified the coding sequence(CDS)of the PRDX1 gene(CeyPRDX1)using reverse transcription PCR(RT-PCR).The CDS sequence was successfully cloned into the pcDNA3.1(+)vector to construct an expression vector and named pcD-NA3.1(+)-CeyPRDX1.The pLJM1-EGFP Lentivirus vector with EGFP fluorescent label was used to construct overex-pression vector of CeyPRDX1(pLJM1-CeyPRDX1-EGFP),and its subcellular localization was observed in the cyto-plasm and nucleus of human HaCaT cells.The results of homology comparison and phylogenetic tree analysis indicated that CeyPRDX 1 has the highest homology and the closest phylogenetic relationship with Eastern European red deer(Cer-vus elaphus)and red deer(Cervus canadensis).Bioinformatic analysis revealed that CeyPRDX 1 is a conserved,hydro-philic protein without N-glycosylation or transmembrane regions,and interacts with various antioxidant proteins.This study provides a foundation for future functional research on the CeyPRDX1 gene.