卤地菊水煎液保护脓毒症大鼠急性肺损伤的实验观察OA

Objective:To observe the protective effect of the aqueous decoction of Ludiju against acute lung injury(ALI)in septic rats and explore its mechanism of action.Methods:Seventy male Sprague-Dawley rats were randomly di-vided into 6 groups using a random number table:sham operation group,model group,positive drug group,and Ludiju low-dose,medium-dose,and high-dose groups.Rats in the sham operation and model groups were given distilled water(10 ml/kg)by gavage daily.Rats in the administration groups were given dexamethasone(0.05 mg/kg)or Ludiju aqueous decoction(3.15 g/kg,6.30 g/kg,12.0 g/kg)by gavage,once daily for 4 consecutive days.Three hours after the last admin-istration,the sepsis ALI model was established using cecal ligation and puncture(CLP).The mortality rate of the rats was observed 18 hours after surgery.The lung wet/dry weight ratio(WW/DW)of the surviving rats was calculated to observe the pathological morphology of lung tissue.The serum levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),in-terleukin-18(IL-18)and the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)were detected.The right lung was collected for pathological observation and to detect the protein expression of NLRP3,TXNIP and Caspase-1.Results:Compared with the model group,the mortality rate decreased in all administration groups,with the Ludiju high-dose group having the lowest mortality rate(33%).The lung WW/DW ratio decreased in all administration groups,and the differences were statistically significant(P<0.01).Neutrophil infiltration,alveolar wall thickening and solidation,pulmo-nary interstitial edema and hemorrhage were alleviated in the treated groups..Compared with the model group,serum levels of TNF-α,IL-1βand IL-18 decreased in the Ludiju low-,medium-,and high-dose groups;TNF-α and IL-18 levels de-creased in the positive drug group;SOD activity increased in the Ludiju low-and high-dose groups;GSH-Px activity in-creased in the Ludiju medium-and high-dose groups;SOD activity decreased and GSH-Px activity increased in the posi-tive drug group;and the protein expression of NLRP3,TXNIP and Caspase-1 decreased in the positive drug group and all Ludiju dose groups.The differences compared with the model group were statistically significant(P<0.05,P<0.01).Conclusion:The aqueous decoction of Ludiju has a protective effect on rats with ALI induced by sepsis.Its mechanism may be related to inhibiting the inflammatory response,enhancing antioxidant capacity,and regulating proteins associated with the TXNIP/NLRP3 pathway.