CST1在肝内胆管癌中的表达变化及其对肿瘤细胞恶性生物学行为的影响OA

Objective To observe the expression pattern of Cystatin 1(CST1)in the intrahepatic cholangiocarcinoma(ICC)tissues and to investigate its effects on the malignant biological functions of ICC cells.Methods We collected samples of ICC tissues,adjacent tissues and tumor junction tissues.Transcriptome sequencing was performed to analyze the differential expression of CST1 mRNA.Immunohistochemistry(IHC)was employed to validate the protein expression levels of CST1.Furthermore,the correlation between CST1 mRNA expression and the prognosis of patients with ICC was analyzed using The Cancer Genome Atlas(TCGA)database.ICC cells were divided into the experimental group(treated with exoge-nous recombinant CST1 protein)and control group(without CST1 treatment).The cell proliferation ability was assessed u-sing CCK-8 assay,while the cell migration ability was evaluated using Scratch wound healing and Transwell migration as-says.Results Both transcriptome sequencing and IHC showed that CST1 mRNA and protein expression levels were signifi-cantly higher in ICC tissues than in the adjacent and junction tissues(all P＜0.05).Analysis of the TCGA database indica-ted that high CST1 expression was significantly correlated with poor prognosis in ICC patients(P＜0.05).In vitro experi-ments demonstrated no statistically significant difference in cell proliferation between the two groups(P＞0.05).However,the cell migration rates of the experimental group were higher than those of the control group at 12 and 24 h in the Scratch wound healing assay,and more cells migrated through the Transwell membrane in the experimental group than in the control group(all P＜0.05).Conclusion CST1 is up-regulated in ICC and serves as a potential biomarker for poor prognosis,and it may play a crucial role in the progression of ICC by promoting cancer cell migration.