高浓度Zebularine诱发小偃麦染色体变异研究OA

Chemical mutagenesis is one of the important approaches to induce plant trait variation.Cur-rently,the most commonly used chemical mutagen is ethyl methyl sulfonate(EMS).However,there are few reports on the use of cytosine analog Zebularine for induction and selection of superior germplasm resources in cereal crops.In this study,high concentration of Zebularine was used as the mutagen to treat germinated seeds of three Trititrigia lines(XY78829,XY7430,and XY96253).The results showed that abundant chromosomal structural variations were observed in the metaphase cells of Trititrigia root tips compared with the negative control(ddH2O),mainly including chromosomal translocations(429,59.0％)and deletions or fragments(298,41.0％).The frequency of intergenomic translocations(380,52.3％)was significantly higher than that of intragenomic translocations(49,6.7％).Within intergenomic translocations,the most translocations oc-curred between homeologous group A and B(111,15.3％).These conclusions could provide valuable infor-mation for further transfer and utilization of beneficial genes from Thinopyrum.