石笔木不同部位提取物体外降血糖功效的比较OA

Objective To explore the α-glucosidase inhibitory activities of the extracts of different parts of Tutcheria austrosinica,the ethanol extracts and water extracts of the flowers,stamens,leaves,pericarps and seeds of T.austrosinica were prepared by refluxing extraction.Methods The α-glucosidase inhibitory activities of 10 kinds of extracts were screened with 4-nitrophenyl-D-glucopyranoside(PNPG)as substrate,the kinetics of active extracts was studied by enzyme-catalyzed reaction,and the Lineweaver-Burk curve was drawn to deduce their inhibitory types.Results The extracts of flowers,stamens,pericarps and seeds showed potent α-glucosidase inhibitory activities,and the inhibitory activity of the extract from the stamens was relatively high,with an IC50 value of 1.30 μg·mL-1.Inhibition dynamics analysis showed that the extracts of flowers,stamens,pericarps and seeds were all close to mixed modes of inhibition against α-glucosidase.Conclusion The extracts of flowers,stamens,pericarps and seeds of T.austrosinica exhibited promising α-glucosidase inhibitory activities.