牛蒡根总多酚提取工艺以及含量测定OA

Objective To optimize the extraction process of total polyphenols from Arctium lappa L.roots,establish a purification and enrichment method,and determine the content of its characteristic marker components,so as to provide a basis for subsequent research.Methods With total polyphenol content(TPC)as the evaluation index,single-factor experiments were conducted to screen the optimal parameters of ethanol concentration,solid-to-liquid ratio,and extraction temperature.The crude extract was purified and enriched by sequential petroleum ether defatting,n-butanol extraction,and AB-8 type macroporous adsorption resin.Folin-Ciocalteu method was used to determine the TPC,and high-performance liquid chromatography(HPLC)was applied for the quantitative analysis of 7 marker phenolic acid components.Results The optimal extraction conditions were 70％ethanol,a solid-to-liquid ratio of 1∶15(g∶mL),and extraction at 60 ℃,under which the TPC of the crude extract reached 2.71％.After purification and enrichment,the TPC was significantly increased to 59.84％.HPLC analysis showed that the total content of 7 marker components(including neochlorogenic acid and chlorogenic acid)accounted for 28.05％of the purified extract.Conclusion The established extraction and enrichment processes are efficient and feasible,and the main component contents of the extract are clarified,which provides technical support for the development and utilization of Arctium lappa L.root polyphenols.