黔北黑猪不同骨骼肌内脂肪沉积相关lncRNAs的鉴定OA

This study aims to explore the candidate lncRNAs related to intramuscular fat deposition in different types of skeletal muscle of Qianbei black pigs.Nine healthy female Qianbei black sows(180±11 days old and 60±7 kg in weight)with close kinship are randomly divided into 3 groups.Samples are collected from the longissimus dorsi and psoas major muscles.RNA-seq is used to construct lncRNA expression profiles,and differentially expressed lncRNAs are screened and validated by qRT-PCR.Bioinformatics methods are employed to predict the target genes of these differentially expressed lncRNAs,followed by GO and KEGG functional enrichment analyses to identify lncRNAs related to intramuscular fat deposition.Additionally,an lncRNA-mRNA regulatory network is constructed.A total of 4 232 lncRNAs are annotated in the two skeletal muscles of Qianbei black pigs,from which 45 differentially expressed lncRNAs are identified(23 downregulated and 22 upregulated).The qRT-PCR results indicate that the expression patterns are highly consistent with the RNA-seq data.GO and KEGG analysis reveal that the target genes of differentially expressed lncRNAs are mainly enriched in GO terms such as phospholipid,lipid and fatty acid transport,fatty acid breakdown,and negative regulation of estrogen receptor signaling pathways,as well as signaling pathways involving ABC transporters,endocytosis,bile secretion,metabolic pathways,and peroxisomes.The lncRNA-mRNA network indicates that TCONS_00123816 and TCONS_00014197 may regulate intramuscular fat deposition through the PPAR signaling pathway and the Wnt signaling pathway,respectively.TCONS_00123816 and TCONS_00014197 may regulate skeletal muscle fat deposition through their target genes in pig.These findings provide data support for in-depth analysis of the mechanisms of fat deposition in different types of skeletal muscle and hold significant implications for assisting in the breeding of high-quality meat varieties.