白及块茎有效成分化学计量评价标准及与抗氧化活性相关性分析OA

[Objective]This study aimed to evaluate the quality of Bletilla striata tubers from different re-gions and ages using stoichiometric methods,and to establish stoichiometric grading criteria for the ac-tive components in B.striata tubers.[Method]The B.striata tubers of 1,2,and 3 years of age col-lected from Yunyan District(Y),Bozhou District(B),and Shibing County(S)were used as research materials.The contents of Bletilla polysaccharide(P),militari(M),total phenols(TP),and antioxi-dant activity were determined.Stoichiometric methods were employed to investigate the stoichiometric characteristics of three active components in B.striata.[Result]Differences in the contents,stoichio-metric characteristics,and antioxidant activity of active components were observed among B.striata tu-bers from different regions and ages.The Bletilla polysaccharide content across different ages followed the order:1 a>2 a>3 a.Total phenols,stoichiometric characteristics of active components,and antioxi-dant activity were highest in 3-year-old tubers from Shibing County.The militari content in tubers from Yunyan District and Bozhou District followed the order:3 a>2 a>1 a,with the highest militari content found in 2-year-old tubers from Shibing County.Polysaccharide content was negatively correlated with the stoichiometric characteristics of active components and antioxidant activity,while total phenol con-tent was positively correlated with these parameters.Based on cluster analysis,the stoichiometric charac-teristics of active components were classified into three grades.[Conclusion]The stoichiometric ratio of active ingredients in Bletilla striata tubers mainly depends on polysaccharides and total phenols,better antioxidant activity was indicated by a larger ratio of active component stoichiometric characteristics of Bletilla striata.Stoichiometric evaluation has important guiding significance for the quality evaluation of Bletilla striata tuber.