有色玉米籽粒花青素成分解析及分类方法建立OA

[Objective]This study aims to establish a classification system for pigmented maize kernels and develop a quantitative classification framework to improve the efficiency of anthocyanin detection and the accuracy of variety screening.[Method]A total of 212 anthocyanin-rich homozygous maize inbred lines were used as materials.Kernel color phenotypes were observed via visual inspection and color cards,while qualitative and quantitative analyses of anthocyanin components were performed using high-performance liquid chromatography(HPLC)to precisely investigate the correlation between color charac-teristics and anthocyanin composition.[Result]The core anthocyanin components in the kernels of pig-mented sweet and waxy maize inbred lines were cyanidin and pelargonidin.The total anthocyanin content in sweet maize was 718.38 mg/kg,which was 1.07 times higher than that in waxy maize.Significant differ-ences were observed between the two types in the detection rate of peonidin and the contribution rate of cyanidin.The anthocyanin content in kernels grown in Yunnan was 8.31%higher than that in Sichuan.A total of 25 germplasm materials with high anthocyanin content were selected,among which the waxy maize line 24CH92 exhibited the highest content at 2,884.09 mg/kg.The 212 inbred lines were classified into 48 groups.The PANTONE 229 U group showed the highest anthocyanin content at 723.43 mg/kg,and RGB values were significantly negatively correlated with anthocyanin content.[Conclusion]An effi-cient and low-cost prediction system for anthocyanin content was established based on the RGB model of kernel color.The quantitative relationship between kernel color and anthocyanin composition was clari-fied,providing a practical technical pathway for the precision breeding and germplasm innovation of pig-mented fresh-eating maize.