茄子单倍体育种研究进展OA

Eggplant(Solanum melongena L.)is widely cultivated around the world as a vital vegetable crop,however,its conventional breeding techniques are the time-consuming and relatively ineffective.Haploid breeding technology is served as one of the effective technical approaches for rapidly obtaining double haploid(DH)lines,thus enabling significant shortening of breeding cycle and facilitating the development of elite germplasm.The technology of haploid breeding mainly includes anther culture and microspore culture.In this paper,the progresses of eggplant anther culture and microspore culture in recent years are reviewed,focusing on the effects of genotype,microspore development stage,pretreatment conditions,medium and hormone combination,and medium additives on eggplant callus and embryo production.Furthermore,the main problems existing in the current research and the prospects for the application of eggplant haploid technology are analyzed,including high contamination rates during the cultivation process,low embryogenesis rates with significant genotype dependence,and an excessively long embryoid-to-plantlet cycle.This review will provide useful references for the development of haploid breeding technology in eggplant.