靶向普通马铃薯疮痂病的拮抗链霉菌筛选、复配组合及防治效果OA
Strain Screening,Combinations Preparation and Biocontrol Efficacy of Antagonistic Streptomyces spp.Targeting Potato Common Scab
马铃薯(Solanum tuberosum)疮痂病由致病链霉菌(Streptomyces spp.)引起,是一种世界范围内发生的重要土传病害.非致病链霉菌能够产生大量具有抗菌促生作用的代谢物,是最具生防潜能的微生物之一.为获得对普通马铃薯疮痂病具有生防潜力的非致病链霉菌,本研究开展了皿内抑菌活性筛选、拮抗链霉菌复配组合配置、拮抗链霉菌复配组合抑菌活性测试、组合中菌株分子鉴定及抑菌促生特性分析、温室盆栽防效评价等研究.结果表明:自77株链霉菌中获得15株对普通马铃薯疮痂病致病菌—疮痂链霉菌(Streptomyces scabies)菌株LBX-7有明显拮抗作用的菌株,其中菌株ZL21的抑菌带最宽(8.17 mm).经菌株相容性测试和复配组合配置,获得 3 个对 LBX-7 有拮抗作用的组合,其中组合 C(ZL21\ZL109\ZL144)的抑菌效果最佳,抑菌带宽度7.67 mm.多基因测序鉴定组合中的菌株,确定菌株ZL21 和 ZL144 为橄榄链霉菌(S.olivaceus);ZL54、ZL68、ZL79、ZL90、ZL109 和 05B3 为小链霉菌(S.parvus).组合中所有菌株均具有产生蛋白酶和几丁质酶、分解钾和固定氮的能力,均不能够溶解无机磷,个别菌株能够产生吲哚乙酸、纤维素酶、漆酶和淀粉酶.温室盆栽防治实验表明,单个菌株中,ZL21的防效最高(75.2%),3个组合中,C的防效最佳(54.4%).本研究获得了针对普通马铃薯疮痂病具有较好防效的链霉菌菌株ZL21,作为普通马铃薯疮痂病生防资源具有较好的开发应用潜力.
Potato(Solanum tuberosum)common scab,caused by pathogenic Streptomyces spp.,is an important soil-borne disease that occurs worldwide.Non-pathogenic Streptomyces can produce a large number of metabolites with antimicrobial and growth-promoting effects,making them one of the microorganisms with the greatest biocontrol potential.To obtain non-pathogenic Streptomyces strains with biocontrol potential against Streptomyces scabies which causes potato common scab,this study conducted series of assays,including screening for antimicrobial activity of single strain in vitro,preparation for antagonistic Streptomyces combinations,testing for antimicrobial activity of the combinations,molecular identification of strains included in the different combinations,analysis for their antimicrobial and growth-promoting characteristics,and evaluation for biocontrol efficacy in greenhouse.The results showed that 15 strains with significant antagonistic effects against the S.scabies strain LBX-7 were obtained from 77 non-pathogenic Streptomyces strains.Among them,strain No.ZL21 exhibited the widest inhibition band(8.17 mm).After strain compatibility testing and combinations preparation,3 combinations with antagonistic effects against LBX-7 were obtained.Among these,combination No.C(ZL21\ZL109\ZL144)showed the best antimicrobial effect,with an inhibition band of 7.67 mm in width.Multi-gene sequencing were used to identify the strains in the 3 combinations,strains No.ZL21 and ZL144 belonged to S.olivaceus,while strainsZL54,ZL68,ZL79,ZL90,ZL109 and 05B3 were identified as S.parvus.All strains in the combinations had the abilities to produce protease and chitinase,decompose potassium and fix nitrogen,but none of them could dissolve inorganic phosphorus.Several strains were capable of producing indole-3-acetic acid,cellulase,laccase and amylase.Greenhouse pot control experiment indicated that ZL21 exhibited the highest biocontrol efficacy(75.2%)in all the tested single strains,and combination No.C showed the best efficacy(54.4%)among the 3 combinations in greenhouse.This study obtained candidate Streptomyces strains ZL21 with good biocontrol efficacy against potato common scab.This strain exhibits promising potential as resources for controlling potato common scab.
陈辰;张才华;王世一;张昊玉;丁毓;雷博超;孙蔓莉;赵伟全;于秀梅
河北农业大学 生命科学学院/河北省植物生理与分子病理学重点实验室,保定 071001河北农业大学 生命科学学院/河北省植物生理与分子病理学重点实验室,保定 071001河北久恩农业开发有限公司,张家口 076550河北久恩农业开发有限公司,张家口 076550河北农业大学 植物保护学院/河北省农作物病虫害生物防治技术创新中心,保定 071001河北农业大学 植物保护学院/河北省农作物病虫害生物防治技术创新中心,保定 071001河北农业大学 植物保护学院/河北省农作物病虫害生物防治技术创新中心,保定 071001河北农业大学 植物保护学院/河北省农作物病虫害生物防治技术创新中心,保定 071001河北农业大学 生命科学学院/河北省植物生理与分子病理学重点实验室,保定 071001
农业科技
生防链霉菌马铃薯疮痂病抑菌活性促生能力生防效果
Biocontrol StreptomycesPotato common scabAntimicrobial activityGrowth-promoting abilityBiocontrol efficacy
《农业生物技术学报》 2026 (5)
1118-1131,14
河北省中央引导地方科技发展资金(246Z6503G)河北省自然科学基金(C2025204083)石家庄市驻冀高校基础研究项目(241791087A)
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