基因聚合培育兼具优质和抗条锈病小麦新品种OA
Breeding of New Wheat(Triticum aestivum)Cultivar Using Gene Pyramiding Method for High-quality and Stripe Rust Resistance
抗病、优质、高产协同改良一直是小麦(Triticum aestivum)育种的重要内容,培育兼具抗条锈病和优质的旱地高产小麦新品种对发展甘肃现代寒旱农业尤为重要.本研究以含有已知优质麦谷蛋白亚基基因Ax1/Ax2*、Bx7+By8和多效性抗条锈病基因Lr34/Yr18/Pm38/Sr57、Lr27/Yr30/Sr2/Sb3的太谷核不育小麦轮回群体选择的品系'Ta04-44-1-3'为母本,含有已知优质麦谷蛋白亚基基因Bx7+By8、Bx7+By9和Glu-A3d的'兰天19号'为父本进行杂交组配,利用优质麦谷蛋白亚基基因和抗条锈病基因的特异性分子标记,结合抗病性、品质鉴定及田间综合性状选择,育成兼具优质、抗条锈病、耐逆丰产的旱地冬小麦新品种'兰天214'.该品种含有优质麦谷蛋白亚基基因Ax1/Ax2*、Bx7+By8、Bx7+By9、Glu-A3d和多效性抗病基因Lr34/Yr18/Pm38/Sr57和Lr27/Yr30/Sr2/Sb3.抗病性鉴定结果表明,其苗期对条锈混合菌表现为中度感病(moderately susceptible,MS),成株期对条锈混合菌为免疫;品质测试结果表明,其籽粒容重为829 g/L,蛋白质含量为13.4%,湿面筋含量为33.2%,稳定时间6.8 min,吸水率58.8%,最大拉伸阻力426 EU,拉伸面积92 cm2,达到中强筋小麦标准.冬小麦新品种'兰天214'的育成可为甘肃陇东南山旱地生产提供优质抗条锈病冬小麦新品种,也为今后该区域开展多基因聚合育种提供新的亲本资源.
Improvement of disease resistance,quality,and high yield simultaneously has been a long-standing goal in wheat(Triticum aestivum)breeding.Breeding new high-yielding dryland wheat varieties with both stripe rust resistance and high quality is particularly important for developing modern cold-arid agriculture in Gansu province.In this study,'Ta04-44-1-3'wheat line developed from recurrent selection population selection of Taigu genic male-sterile wheat carrying the known high-quality glutenin subunit genes Ax1/Ax2* and Bx7+By8,as well as the pleiotropic stripe rust resistance genes Lr34/Yr18/Pm38/Sr57 and Lr27/Yr30/Sr2/Sb3 was utilized as the female parent,and'Lantian 19',which possessed the known high-quality glutenin subunits Bx7+By8,Bx7+By9,Glu-A3d,as the male parent for hybridization.Using specific molecular markers for the high-quality glutenin subunit and stripe rust resistance genes,combined with disease resistance evaluation,quality assessment,and field-based comprehensive trait selection,a new winter wheat variety named'Lantian 214'was developed.This variety combines high-quality,stripe rust resistance,stress tolerance,and high yield,making it suitable for dryland conditions.The variety possessed the high-quality glutenin subunits Ax1/Ax2*,Bx7+By8,Bx7+By9,Glu-A3d,as well as the pleiotropic resistance genes Lr34/Yr18/Pm38/Sr57 and Lr27/Yr30/Sr2/Sb3.Disease resistance evaluation revealed that the variety was moderately susceptible(MS)to mixed races of stripe rust at the seedling stage but exhibited immunity to prevalent mixed races at the adult plant stage.Grain quality analysis indicated that its grain test weight was 829 g/L,protein content was 13.4%,wet gluten content was 33.2%,dough stability time was 6.8 min,water absorption rate was 58.8%,maximum resistance to extension was 426 E.U,and extensibility area was 92 cm2,meeting the standards for medium-strong gluten wheat.The development of new winter wheat cultivar'Lantian 214'provides a high-quality,disease-resistant winter wheat variety suitable for dryland production in the mountainous southeastern Gansu.It also offers new parental germplasm for future efforts in pyramiding multiple genes for breeding in this region.
何瑞;马瑞;郭莹;杜久元;张礼军;展宗冰;王红梅;刘风;白斌
甘肃省农业科学院 小麦研究所,兰州 730070甘肃省农业科学院 小麦研究所,兰州 730070甘肃省农业科学院 小麦研究所,兰州 730070甘肃省农业科学院 小麦研究所,兰州 730070甘肃省农业科学院 小麦研究所,兰州 730070甘肃省农业科学院 小麦研究所,兰州 730070甘肃省农业科学院 生物技术研究所,兰州 730070甘肃省农业科学院 科研管理处||兰州 730070甘肃省农业科学院 小麦研究所,兰州 730070
农业科技
小麦条锈病抗性优质分子标记辅助选择基因聚合
WheatStripe rust resistanceHigh-qualityMolecular marker assisted selectionGene pyramiding
《农业生物技术学报》 2026 (5)
1048-1058,11
国家重点研发计划(SQ2022YFD1200003)甘肃省重点研发计划(25YFNA008)甘肃省科技重大专项(25ZDNA001)
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