QuEChERS-超高效液相色谱-串联质谱法测定蔬果中溴菌腈的残留方法OA

In this study,a QuEChERS-ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was developed to determine bromothalonil residues in a variety of fruits and vegetables.Bromothalonil was extracted from samples with acetonitrile using vortex oscillation.The organic and aqueous phases were separated by salting out with sodium chloride.The extract was purified with anhydrous MgSO4 and PSA adsorbent and then directly analyzed by UPLC-MS/MS.Determination was carried out using electrospray negative ion mode(ESI-)and multiple reaction monitoring(MRM),and quantification was performed using an external standard method.The results showed that bromothalonil exhibited a good linear relationship over the range of 0.001 to 0.3 mg/L,with correlation coefficients greater than 0.99,meeting the requirements for residue analysis.At spiking levels of 0.01～0.7 mg/kg,the average recoveries ranged from 80.7%to 109%,with relative standard deviations 0.715%～4.41%.This method is simple,accurate and efficient,and is suitable for the determinations of bromothalonil residues in cucumber,citrus,apple and watermelon.