柠条长角象聚集信息素在不同载体中的释放速率及野外防效OA

In order to clarify the release law of aggregation pheromone heptacosane and 2,6,10,14-tetramethylheptadecane in gray rubber stoppers and EP tubes,and provide a technical guidance for the development of green control of attractants for Trigonorhinus sp.The solid-phase microextraction and gas chromatography-mass spectrometry(SPME-GC-MS)were used to study the release rate of single component compounds and binary component compounds in different carriers and different storage temperatures.The daily release amount of binary component compounds was determined by the weighing method,and the attraction effect of the lure was veri-fied in field trapping experiments.The results showed that the release rate of the two compounds showed a high level in the EP tubes under freezing conditions.GC-MS analysis revealed that the binary component reached the maximum release rate on the third day.The weighing method indicated that the release of the compound reached the maximum on the second day.In addition,in the field trapping experiment,it was found that the trapping effect of the binary component was significantly better than that of the single com-ponent.Therefore,it can be concluded that EP tubes were the optimal carrier for the release of aggregation pheromone of Trigonorhi-nus sp.The optimal extraction dose of heptacosane was 100 μg,and the optimal attracting dose was 1 000 μg.The optimal extrac-tion dose of 2,6,10,14-tetramethylheptadecane was 10 μg,and the optimal attracting dose was 100 μg.The optimal extraction time of the two compounds was 0.25 h,and the optimal storage temperature was-20℃.