基于靶向和非靶向筛查技术的农药场地特征污染物筛选研究OACHSSCD

Identifying characteristic pollutants at pesticide-contaminated sites is challenging due to the complex composi-tion and diversity of pesticide residues and their transformation products.To address this issue,this study proposes a novel screening framework that integrates targeted and non-targeted analytical approaches using ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS)and ultra-high-performance liquid chroma-tography-quadrupole time-of-flight tandem mass spectrometry(UPLC-QTOF-MS).A comprehensive scoring system based on 12 evaluation indicators-including structural identification confidence,relative peak area,overall detection frequency,and metabolite identification etc.,was established to improve the accuracy and efficiency of characteristic pollutant identi-fication.A pesticide-contaminated site in Jiangsu Province was selected as a case study.Targeted analysis detected nine pesticides,while non-targeted analysis identified eleven pesticides and related metabolites.These include six fungicides(carbendazim,azoxystrobin,pyraclostrobin,thiophanate-methyl,tebuconazole,and difenoconazole),one metabolite(benzimidazole),three herbicides(atrazine,pretilachlor,and flumioxazin),and one insecticide(paclobutrazol).Based on the proposed framework,carbendazim,atrazine,and tebuconazole were identified as the characteristic pollutants at the study site.This approach provides a practical strategy for the systematic identification of characteristic pollutants at pesti-cide-contaminated sites.