铁皮石斛多糖对高糖诱导的视网膜毛细血管周细胞凋亡的影响OA

·AIM:To investigate the protective effects of Dendrobium officinale polysaccharide(DOP)on high glucose-induced apoptosis in retinal capillary pericytes and its potential mechanism involving mitochondrial function. ·METHODS:Retinal capillary pericytes were allocated into five groups:normal control(NC),high glucose(HG),and three DOP treatment groups(low,DOP-L;medium,DOP-M;high,DOP-H).Pericyte ultrastructure was analyzed using transmission electron microscopy(TEM).Apoptotic rate was quantified via Annexin V-FITC staining.Mitochondrial transmembrane potential was assessed using the JC-1 probe.Quantitative real-time polymerase chain reaction(qRT-PCR)and Western blot were employed to measure expression levels of cytochrome C(Cyt C),B-cell lymphoma 2(Bcl-2),Bcl-2-associated X protein(Bax),Caspase-9,and Caspase-3,respectively. ·RESULTS:Compared to the NC group,pericytes exposed to HG exhibited significant mitochondrial damage,elevated apoptotic rate,increased mRNA and protein expression of Cyt C,Bax,Caspase-9,and Caspase-3(all P<0.01),alongside a marked reduction in mitochondrial transmembrane potential and expression of Bcl-2 mRNA and protein(all P<0.01).In contrast,DOP treatment groups(DOP-M,DOP-H)dose-dependently ameliorated mitochondrial damage,reduced apoptotic rate,downregulated Cyt C,Bax,Caspase-9,and Caspase-3 expression,enhanced mitochondrial transmembrane potential,and upregulated Bcl-2 expression relative to the HG group(all P<0.05). ·CONCLUSION:DOP attenuates high glucose-induced apoptosis and mitochondrial injury in retinal capillary pericytes.The underlying mechanism may involve the restoration of mitochondrial transmembrane potential.