微生物菌剂配施对小叶杨生理特性及土壤理化性质的影响OA

[Objective]The northwestern region of Liaoning Province is located in a wind-water erosion cris-scross zone,where severe soil erosion and continuous decline in soil fertility have significantly con-strained plant growth.In this context,this study aims to investigate the synergistic regulatory effects of dif-ferent microbial inoculant combinations on the physio-ecological characteristics of Populus simonii and the quality of the soil inter-root environment,thereby providing scientific support and technical solutions for ve-getation restoration and ecological rehabilitation in this region.[Methods]Based on a field experiment of Populus simonii Carr.,three microbial inoculants:Arbuscular mycorrhizal fungi(AMF),Trichoderma harzi-anum(TH),and Bacillus mucilaginosus(BM)were aplied using a completely randomized block design.A total of eight different treatments were established:AMF,TH,BM,AMF+BM,AMF+TH,TH+BM,AMF+TH+BM,and a non-inoculated control(CK).The effects of these treatments on poplar growth were as-sessed,together with changes in soil aggregate stability,mechanical composition,physicochemical prop-erties,and available nutrient content.[Results](1)All treatments improved poplar growth compared with the control,with the TH+BM treatment exhibiting the strongest synergistic effect.Relative to CK,plant height,stem thickness and net photosynthetic rate of poplar increased by 50%,51.75%and 65.38%,re-spectively,and the plant growth index reached a peak value of 0.90.(2)Soil aggregate analysis indicated that particles sized 0.25-0.05 mm were the dominant fraction.In May,the AMF+TH treatment produced the highest sand content(58.06%).During May and October,the soil texture under the sole application of AMF shifted from sandy to silty soil,while the soil under the AMF+BM treatment changed from silty to sandy soil.(3)The AMF+TH treatment most effectively increased soil organic matter in May,while TH+BM treatment had the greatest improvement in soil pH.In October,TH+BM treatment significantly en-hanced total nitrogen,total phosphorus,and total potassium improvement by 58.82%,90.91%,and 17.31%,respectively,compared with CK.Regarding available nutrients,AMF+TH treatment most effect-ively increased alkaline nitrogen,TH+BM treatment maximized available potassium,and AMF+TH+BM(May)and AMF+BM(October)produced the greatest increases in available phosphorus.Soil quality in-dex(SQI)analysis indicated that TH+BM had the strongest overall improvement effect,with a peak SQI value of 0.85,representing a 1.58-fold increase relative to CK.[Conclusion]The combined application of Trichoderma harzianum and Bacillus mucilaginosus(TH+BM)exerts pronounced synergistic effects on promoting plant growth and improving soil quality.This microbial strategy effectively alleviates constraints on vegetation development and soil degradation in wind-water erosion-prone areas,providing an efficient and sustainable biotechnological approach for ecological restoration in such fragile environments.