沙棘绕实蝇响应滞育转录组及RboHSP70基因表达分析OA

[Objective]This study aimed to explore the genes associated with diapause in Rhagoletis batava obseuriosa Kol.and to elucidate the molecular mechanisms underlying its diapause regulation.[Methods]Transcriptomic analysis was performed using RNA-Seq technology on two types of pupae:emerging pupae(EP,capable of eclosion within the year)and dispause pupae(DP,incapable of eclosion within the year).Differential expression of the RboHSP70 gene family during diapause was further investig-ated.[Results]Comparative transcriptomic analysis between EP and DP identified 25,538 differentially ex-pressed genes(DEGs)of which 18,437 genes were down-regulated and 7,101 genes were up-regulated in DP compared to EP.GO functional annotation and KEGG pathway analysis revealed significant enrich-ment of DEGs in stress response,protein folding,and metabolic pathways.Heat shock proteins(HSP70s)were highlighted as key diapause regulators.Further characterization of the HSP70 family identified 20 diapause-associated members(RboHSP70-1 to RboHSP70-20).Physicochemical characterization showed an average molecular weight of 71.82 kDa and an isoelectric point(pI)of 5.49.Phylogenetic ana-lysis classified these members into five groups(Group I-V).while motif analysis indicated that most se-quences contained 17 conserved motifs,Transcriptional profiling demonstrated that 16 RboHSP70 genes were significantly up-regulated in diapause pupae,with RboHSP70-13 showing the most pronounced up-regulation,suggesting its pivotal role in diapause regulation.[Conclusion]This study provides molecular and theoretical foundations for identifying and functionally characterizing key genes involved in diapause regulation in Rhagoletis batava obseuriosa.