沙棘MADS-MIKC基因家族的全基因组鉴定及表达分析OA

[Objective]This study aimed to identify members of the MADS-MIKC gene family in sea buck-thorn,and to analyze their physicochemical properties,evolutionary relationships,gene structures and ex-pression patterns,thereby providing a molecular basis for breeding high-quality sea buckthorn germplasm resources and elucidating regulatory mechanisms underlying fruit development.[Method]Using the labor-atory's sea buckthorn genome data,bioinformatics methods were used to identify the members of the gene family,and combined with transcriptome sequencing and RT-qPCR to verify the expression patterns of the genes during the development of sea buckthorn pulp and seeds.[Result]A total of 55 MADS-MIKC gene family members were identified in the sea buckthorn genome and divided into 11 subfamilies,all genes contained introns.Promoter analyses indicated that most genes harboured multiple cis-acting elements as-sociated with responses to abscisic acid,auxin,and gibberellin.The MADS-MIKC gene family was un-evenly distributed across 12 chromosomes,and segmental duplication appeared to be the primary driver of family expansion.Expression heatmap and RT-qPCR analysis showed that the gene family exhibited signi-ficant expression differences in different developmental stages and tissues:the SEP and SHP subfamilies were highly expressed in pulp and seeds,and RT-qPCR verified the reliability of the transcriptome data.[Conclusion]The sea buckthorn MADS-MIKC gene family has tissue-and stage-specific regulatory roles in the process of fruit development,which provides a theoretical basis for analyzing the fruit ripening mech-anism of sea buckthorn and molecular breeding.