柳杉毛虫OBP基因家族的鉴定、进化与功能分析OA

[Objective]Odorant Binding Proteins(OBP)are the first key proteins involved in the insect ol-factory sensory pathway,responsible for binding and transporting odorant molecules to olfactory receptors.Dendrolimus houi Lajonquiere is an important foliage-feeding pest in southern China,and investigated of its OBP gene family is essential for understanding larval foraging behavior as well as adult recognition and oviposition site selection,thereby providing a molecular basis for pest control strategies.[Methods]In this study,using the OBPs of the model species,Bombyx mori,were identified from D.houi and its closely re-lated species D.punctatus.Phylogenetic relationships were reconstructed,and the characteristics of OBPs of D.houi,including motifs,physicochemical properties,transmembrane spiral region,secondary and ter-tiary structures were analyzed.In addition,the expression levels of OBPs of D.houi at different develop-mental stages were explored.[Results]A total of 41 OBPs were identified in D.houi and were evolutionar-ily grouped into six subfamilies:PBP/GOBP,ABPⅠ,ABPⅡ,Plus-C,Minus-C,and CRLBP.Sequence structures of members of different subfamilies of OBPs were distinctly differentiated,and members of the same subfamily had similar biochemical and structural properties.The nature and structure of a few mem-bers were different from members of same subfamilies.The OBP gene family was classified into six cat-egories of genes based on differences in their expression across six developmental stages of D.houi.The class Ⅰ,Ⅱ,and Ⅲ genes were specifically expressed during the larval stage(instars 1～3,4～5,and 6～7),class Ⅳ genes were specifically expressed during the pupal stage,class Ⅴ genes were highly ex-pressed in the adult stage,and class Ⅵ genes were highly expressed in both the egg and adult stages.[Conclusion]The OBPs of D.houi can be divided into six subfamilies,with diverse structural features and expression patterns,suggesting functional diversification.In addition to olfactory-related functions,certain OBPS may also be involved in regulating growth,development and reproduction.The study provides a mo-lecular basis for resolving the mechanism of OBP-associated olfaction in D.houi.