肺不藏魄不寐模型大鼠相关脏器Bcl-2、Bax、Caspase-3表达水平研究OA

Objective To investigate the expression levels of Bcl-2,Bax and Caspase-3 in the related organs of insomnia rats with lung not storing corporeal soul,so as to provide a basic experimental basis for the traditional Chinese medicine(TCM)classification of the five spirits of insomnia.Methods According to the random number table method,16 SPF male SD rats were di-vided into large platform control group and lung not storing corporeal soul group,with 8 rats in each group.The large platform con-trol group was used to establish the water environment large platform method,and the lung not storing corporeal soul group was used to establish the model by the water environment small platform method.The general condition of the rats in the two groups was monitored and the water maze experiment was performed.HE was used to observe the pathological changes of lung tissues of the two groups,and immunohistochemistry(IHC)method and Western Blot(WB)were used to detect the expression levels of Caspase-3,Bcl-2 and Bax in the brain and lung tissues of the two groups.Results Compared with those in the control group,the general situation showed that the rats in the lung not storing corporeal soul group gradually became depressed,their hair was dull,there was hair loss from time to time,and their diet gradually deteriorated(P＜0.05).HE staining showed disordered alveolar structure,alveolar epithelial cell edema,widened alveolar septum and more lymphocyte and plasma cell infiltrates.IHC method showed that the expression of Bel-2 in brain tissue and lung tissue decreased(P＜0.05),and the expressions of Bax and Caspase-3 increased(P＜0.05).WB results showed that the expression of Bcl-2 in brain tissue and lung tissue in lung tissue was significantly decreased(P＜0.01),the expressions of Bax and Caspase-3 increased(P＜0.05).Conclusion The changes of apoptosis in brain and lung tissues are related to the occurrence of lung not storing corporeal soul.