负载SNAP的3D打印支架制备及其促成骨作用研究OA

Objective:To develop a S-nitroso-N-acetyl-penicillamine(SNAP)loaded 3D printed scaffold integrating a three-dimensional porous architecture,bioactive signaling,and antibacterial properties,aiming to achieve efficient bone regen-eration while preventing postoperative infections.Methods:Hydrogel scaffolds were fabricated utilizing digital light processing(DLP)technology and loaded with nitric oxide(NO)donor,SNAP.Scaffold morphology and NO release kinetics were subse-quently characterized.Bone marrow mesenchymal stem cells(BMSCs)and MC3T3-E1 cells were employed to evaluate the biocompatibility and in vitro pro-osteogenic capacity of the scaffolds.Furthermore,the antibacterial efficacy of the scaffolds was assessed using the agar plate spreading method.Results:The successfully fabricated SNAP loaded 3D printed scaffolds exhibited a sustained release of NO for at least 3 days.Compared to the control group,these scaffolds effectively inhibited the proliferation of pathogenic bacteria and significantly upregulated the expression of osteogenic genes.Conclusion:This study demonstrates that the SNAP-loaded 3D printed scaffold provides sustained NO release,facilitating simultaneous antibacterial and pro-osteogenic effects.By integrating bone repair capabilities with infection prophylaxis,this dual-functional scaffold holds promising clinical potential for application in bone regeneration.