木质素模型物/CNC的Pickering乳液制备及其抗肿瘤活性OA

Based on the synthesis of lignin dehydrogenation polymer(DHP),an oil-in-water(O/W)Pickering emulsion was prepared using cyclopentanone containing DHP as the oil phase,deionized water as the aqueous phase,and cellulose nanocrystals(CNC)as solid particle stabilizers.Using the Pickering emulsion,a novel drug delivery system was developed.The particle size,stability,and rheological properties of the Pickering emulsion were comprehensively characterized with Fourier-transform infrared spectroscopy(FT-IR),size-exclusion chromatography combined with multi-angle light scattering(SEC-MALS),Malvern laser particle size analyzer,and ultraviolet-visible spectrophotometry.Results showed that the emulsion had an average particle size of approximately 430 nm and exhibited good stability.The drug loading capacity of the Pickering emulsion was 24.7％±2.0％,with an encapsulation efficiency of 80.0％±1.8％.In vitro experiments demonstrated that the DHP-containing CNC-Pickering emulsion remained stable in simulated lumen juice but underwent phase separation(i.e.,emulsion breakdown)in simulated colonic juice.After breakdown,the released DHP was evaluated for antitumor activity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)colorimetric assay.The results indicated significant inhibitory effects against human colon cancer HCT116 cells,with a half-maximal inhibitory concentration(IC50)of 186.97 mg/L.Thus,the lignin model compounds/CNC-based Pickering emulsion enables targeted drug release under enzyme-responsive conditions to achieve the therapeutic effect on colon cancer.