血清miR-92a-3p、SIRT6对胸段食管癌患者术后复发的预测价值OA

Objective To explore the predictive value of serum microRNA(miRNA)-92a-3p and silent in-formation regulator 6(SIRT6)expression levels for post-operative recurrence in patients with thoracic esoph-ageal cancer.Methods A total of 95 patients with thoracic esophageal cancer who underwent radical surgery in the hospital from January 2021 to March 2024 were selected as the study group.Meanwhile,108 healthy volunteers without a history of cancer who had physical examinations in the hospital during the same period were selected as the control group.Baseline data of all subjects on admission were collected by self-made sta-tistical form of the hospital.All patients with thoracic esophageal cancer were followed up for 6 months through regular outpatient reviews after surgery.According to the follow-up results,they were divided into re-currence group and non-recurrence group.The expression level of serum miR-92a-3p was detected by real-time fluorescence quantitative polymerase chain reaction,and the expression level of serum SIRT6 was detected by enzyme-linked immunosorbent assay.Pearson correlation was used to analyze the correlation between serum miR-92a-3p expression level and serum SIRT6 expression level in patients with thoracic esophageal cancer.The receiver operating characteristic(ROC)curve was plotted to analyze the value of serum miR-92a-3p,SIRT6 alone and in combination for predicting post-operative recurrence in patients with thoracic esophageal cancer.Results Compared with the control group,the expression level of serum miR-92a-3p in the study group was higher,and the expression level of serum SIRT6 was lower,with statistically significant differences(P<0.05).There were potential binding sites between miR-92a-3p and SIRT6,and their expression levels were negatively correlated(r=—0.631,P<0.001).In patients with thoracic esophageal cancer,there were statistically significant differences in N stage and TNM stage between patients with different expression levels of serum miR-92a-3p and SIRT6(P<0.05).Following-up results showed that there were 26 patients in the recurrence group and 69 in the non-recurrence group.Compared with the non-recurrence group,the expression level of serum miR-92a-3p in the recurrence group was higher,and the expression level of serum SIRT6 was lower,with statistically significant differences(P<0.05).The results of ROC curve analysis showed that the areas under the curves(AUCs)of serum miR-92a-3p,SIRT6 alone and in combination for predicting post-op-erative recurrence in patients with thoracic esophageal cancer were 0.881,0.867 and 0.971 respectively.The AUC of the combined prediction of the both indicators was significantly larger than that of miR-92a-3p alone(Z=2.417,P=0.015)and SIRT6 alone(Z=2.327,P=0.035).Conclusion The serum miR-92a-3p expres-sion level is high and serum SIRT6 expression level is low in patients with recurrent thoracic esophageal canc-er after surgery,and the combination of the two indicators has a high predictive value for post-operative recur-rence in patients with thoracic esophageal cancer.