血清lncRNA MIR155HG、miR-802联合检测对胃癌淋巴结转移及预后的预测价值OA

Objective To investigate the predictive value of combined detection of serum long non coding RNA(lncRNA)MIR155HG and microRNA-802(miR-802)for lymph node metastasis and prognosis in gas-tric cancer(GC).Methods A total of 106 patients with GC and 80 patients with benign gastric admitted to the Hospital from April 2019 to October 2021 were selected as the GC group and benign gastric disease group respectively,meanwhile,82 healthy volunteers who underwent health examinations at the same hospital during the same period were selected as the control group.Baseline data of GC patients were collected.Based on path-ological staging,GC patients were categorized into non-lymph node metastasis group(N0)and lymph node metastasis group(N1-3).According to the 3-year follow-up results,GC patients were divided into survival group and mortality group.Real-time fluorescent quantitative reverse transcription polymerase chain reaction was used to detect serum lncRNA MIR155HG and miR-802 levels in all subjects.Pearson correlation analysis was performed to assess the relationship between serum lncRNA MIR155HG level and miR-802 level in GC patients.Multivariate Logistic regression analysis was conducted to identify factors influencing lymph node metastasis in GC patients.Receiver operating characteristic(ROC)curves were plotted to evaluate the predic-tive value of serum lncRNA MIR155HG and miR-802 for lymph node metastasis in GC patients.Cox regres-sion analysis was performed to identify factors affecting prognosis in GC patients.Kaplan-Meier survival curves were constructed to analyze the relationship between serum lncRNA MIR155HG,miR-802 levels and prognosis in GC patients.Results Compared with the control group,serum lncRNA MIR155HG levels were elevated and miR-802 levels were decreased in both the GC group and the benign benign gastric disease group,with statistically significant differences(P<0.05).Compared with the benign benign gastric disease group,serum lncRNA MIR155HG levels were elevated and miR-802 levels were decreased in the GC group,with sta-tistically significant differences(P<0.05).Pearson correlation analysis result revealed a negative correlation between serum lncRNA MIR155HG level and serum miR-802 level in GC patients(r=-0.685,P<0.05).lncRNA MIR155HG and miR-802 share specific binding sites.Among 106 GC patients,47 belonged to the non-lymph node metastasis group and 59 to the lymph node metastasis group.Compared with the non-lymph node metastasis group,serum lncRNA MIR155HG level increased in the lymph node metastasis group,and se-rum miR-802 level decreased,with statistically significant differences(P<0.05).Multivariate Logistic regres-sion analysis results showed that elevated serum lncRNA MIR155HG level was a risk factor for lymph node metastasis in GC(P<0.05),while elevated serum miR-802 level was a protective factor for lymph node me-tastasis(P<0.05).ROC curve analysis results revealed that the areas under the curves(AUCs)of serum ln-cRNA MIR155HG,miR-802 alone and in combination for predicting lymph node metastasis were 0.864,0.834 and 0.938 respectively,and the combined AUC of both indicators for predicting lymph node metastasis was significantly larger than that of serum lncRNA MIR155HG or miR-802 alone(Z=2.522,3.290,both P<0.05).Three-year follow-up results revealed 75 patients in the survival group and 31 in the mortality group.Compared with the survival group,the mortality group exhibited a higher proportion of TNM stage Ⅲ+Ⅳdisease,elevated serum lncRNA MIR155HG level and decreased serum miR-802 level,with statistically signif-icant differences(P<0.05).Kaplan-Meier survival curve analysis results revealed that the survival curve of the low-expression group of lncRNA MIR155HG was superior to that of the high-expression group(Log-rank χ2=4.285,P=0.038);the survival curve of the high-expression group of miR-802 was superior to that of the low-expression group(Log-rank χ2=9.424,P=0.002);the survival curve for the early TNM stage group was superior to that of the advanced stage group(Log-rank χ2=8.706,P=0.003).Cox regression analysis results revealed that elevated serum lncRNA MIR155HG level and TNM stage Ⅲ+Ⅳ were risk factors for mortality in GC patients(P<0.05),while elevated serum miR-802 level was a protective factor for mortality(P<0.05).Conclusion Serum lncRNA MIR155HG level is increased in GC patients,while miR-802 level is decreased,and the combined prediction of both indicators demonstrates significant value in predicting lymph node metastasis in GC patients,serving as predictive indicators for lymph node metastasis and prognosis in GC.