基于UHPLC-Q-Exactive-Orbitrap MS/MS和网络药理学探讨金线莲抗抑郁有效成分及作用机制研究OA

Objective:To explore the pharmacological material basis and potential mechanism of action of Anoectochilus roxburghii in the treatment of depression.Methods:Ultra-high performance liquid chromatography-quadrupole-Orbitrap high-resolution mass spectrometry(UHPLC-Q-Exactive-Orbitrap MS)was used to analyze the chemical components and blood components of Anoectochilus roxburghii water extract.The target sites of the blood components were obtained from the PubChem and Swiss Target Prediction databases.The target sites of the disease were obtained from the GeneCards,Therapeutic Target,and OMIM databases.The protein-protein interaction(PPI)network was constructed using the STRING platform.The intersection targets were analyzed for gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment using the DAVID database.Results:A total of 42 compounds were identified in the water extract of Anoectochilus roxburghii,and 14 blood components were identified in the plasma of rats after intragastric administration.There were 265 intersection targets between the disease and the blood components,among which 53 were key targets,mainly involving IL-6,AKT1,SRC,STAT3,PPARG,etc.GO functional enrichment and KEGG pathway analysis showed that Anoectochilus roxburghii may exert its therapeutic effect on depression through pathways such as cancer,regulation of TRP channels by inflammatory mediators,neuroactive ligand-receptor interaction,5-hydroxytryptamine(serotonin)synaptic signaling pathway,VEGF signaling pathway,HIF-1 signaling pathway,PI3K-Akt signaling pathway,and FoxO signaling pathway.Conclusion:The active components such as anoectochiluside,apigenin,and 5-hydroxymethylfurfural in Anoectochilus roxburghii may exert its therapeutic effect on depression through pathways such as cancer,regulation of TRP channels by inflammatory mediators,neuroactive ligand-receptor interaction,5-hydroxytryptamine(serotonin)synaptic signaling,VEGF signaling,HIF-1 signaling,PI3K-Akt signaling,and FoxO signaling,as well asgenes such as IL-6,AKT1,SRC,and STAT3.