14株食药用菌CAZy胞外降解酶分析OA

Phylogenetic relationships and the characteristics of CAZy extracellular degradative enzymes of edible and medicinal fungi,were analyzed based on annotated proteins from 14 strains of edible and medicinal fungi.By combining gene functions with substrate degradation capabilities,the degradation attributes of CAZy extracellular secretory enzymes in relation to substrates such as lignin,cellulose,hemicellulose,pectin,chitin,and starch were inferred.The phylogenetic analysis results showed that truffles and morels belonging to the Ascomycota,clustered into one branch,while the remaining strains belonging to the Basidiomycota clustered into another,being consistent with the fungal classification framework and supporting their early evolutionary status.Analysis of extracellular CAZy degradation enzymes showed that the top three strains with the highest quantity of such enzymes were Auricularia subglabra,A.cornea,and Pleurotus ostreatus,whereas the bottom three were Tricholoma matsutake,Tuber borchii,and T.melanosporum.The arrangement of strains based on the quantity of lignocellulose-degrading enzymes from high to low was:Auricularia subglabra,A.cornea,Volvariella volvacea,Pleurotus ostreatu,Ganoderma sinense,G.leucocontextum,Agaricus bisporus,Flammulina filiformis ym5,Morchella sextelata,Flammulina filiformis wm14,Morchella conica,Tricholoma matsutake,Tuber borchii,and T.melanosporum.This study aims at providing preliminary reference for subsequent development of cultivation substrate formulations and ecological adaptability studies for these edible and medicinal fungi.