腺苷酸基琥珀酸合成酶负介导罗伯茨绿僵菌致病力OA
Adenylosuccinate synthase negatively controls Metarhizium robertsii pathogenicity
腺苷酸基琥珀酸合成酶(adenylosuccinate synthetase,ADSS)在细胞能量代谢与信号传导等过程中具有重要作用,但目前在昆虫病原真菌中的研究未见报道.本研究以罗伯茨绿僵菌Metarhizium robertsii中ADSS家族成员MrADSS-1为研究对象,利用亚细胞定位分析、基因敲除、回补和过表达等方法,系统探究了其在绿僵菌生长、抗逆和致病过程中的作用.结果表明,MrADSS-1 分布在罗伯茨绿僵菌细胞的液泡中.敲除 MrADSS-1 导致菌株在麦芽糖培养基中生长速率显著下降、抗紫外能力减弱,但耐热能力增强.致病力测定结果表明,MrADSS-1 敲除显著提高了绿僵菌附着胞形成率和蝉翅穿透能力,增强了对大蜡螟的毒力,而过表达 MrADSS-1 菌株则致病力下降.本研究结果表明,MrADSS-1 负介导罗伯茨绿僵菌的耐热能力和致病力.
Adenylosuccinate synthetase(ADSS)plays an important role in cellular energy metabolism and signal transduction.However,ADSS had not been reported in entomopathogenic fungi.In this study,function of ADSS family protein MrADSS-1 to the growth,stress resistance and pathogenicity in the entomopathogenic fungus Metarhizium robertsii was characterized by investigating the protein subcellular localization and gene disruption,complementation and over-expression.Results showed that MrADSS-1 is exclusively localized within the vacuole.Disruption of the MrADSS-1 resulted in marked reduction of radial growth of the fungus on maltose medium and a significant decrease in UV-B tolerance,but unexpectedly enhanced thermotolerance.Bioassays revealed that loss of MrADSS-1 led to a pronounced increase in appressorium formation and penetration efficiency through cicada wings,resulting in elevated pathogenicity toward Galleria mellonella larvae.Conversely,over-expression of MrADSS-1 attenuated fungal virulence.These findings demonstrated that MrADSS-1 functions as a repressor of both thermotolerance and virulence in M.robertsii.
王乐陈;郭世悦;赵雨桐;董媛媛;吴文婧;任言;郭复明;王玉龙
安徽农业大学林学与园林学院 重大林业有害生物绿色防控安徽省重点实验室,安徽 合肥 230036安徽农业大学林学与园林学院 重大林业有害生物绿色防控安徽省重点实验室,安徽 合肥 230036安徽农业大学林学与园林学院 重大林业有害生物绿色防控安徽省重点实验室,安徽 合肥 230036安徽农业大学林学与园林学院 重大林业有害生物绿色防控安徽省重点实验室,安徽 合肥 230036安徽农业大学林学与园林学院 重大林业有害生物绿色防控安徽省重点实验室,安徽 合肥 230036安徽农业大学林学与园林学院 重大林业有害生物绿色防控安徽省重点实验室,安徽 合肥 230036涡阳县林业发展中心,安徽 亳州 233600安徽农业大学林学与园林学院 重大林业有害生物绿色防控安徽省重点实验室,安徽 合肥 230036
腺苷酸基琥珀合成酶罗伯茨绿僵菌亚细胞定位逆境胁迫致病力
adenylosuccinate synthaseMetarhizium robertsiisubcellular localizationabiotic stresspathogenicity
《菌物学报》 2026 (4)
134-144,11
合肥市自然科学基金(202312)安徽省大学生创新创业训练计划(S202410364133,X202410364462,X202410364493) This work was supported by the Hefei Municipal Natural Science Foundation(202312)and the Anhui College Student Innovation and Entrepreneurship Training Program(S202410364133,X202410364462,X202410364493).
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