香菇LeATG基因家族及其在菌丝转色过程中的表达OA
Members of the LeATG gene family and their expression during mycelial colouring process of Lentinula edodes
为解析香菇 Lentinula edodes 自噬相关基因(autophagy-related gene,ATG)家族成员在菌丝转色过程中的功能,本研究基于香菇全基因组数据,采用同源比对的方法筛选 LeATG 家族成员,并对其进行生物信息学分析.同时,结合转录组测序数据和RT-qPCR,分析了该家族成员在菌丝转色过程中的表达模式.结果表明,在香菇全基因组中共鉴定出29个LeATG家族成员,属于23类自噬相关基因.染色体定位分析显示,其主要分布于 4 号和 1 号染色体,9 号染色体中未发现分布.LeATG 家族成员基因全长在 595-6 772 bp 之间,LeATG 基因编码蛋白序列长度介于 127-1 998 aa,蛋白相对分子量为 14.8-219.7 kDa,理论等电点为 4.27-9.51,除 LeATG9、LeATG22 和 LeATG27外,其余 24 个 LeATG 蛋白均不含跨膜结构.进化分析结果显示 29 个 LeATG 蛋白聚类为 3 个进化分支,同一类 LeATG 蛋白分散于不同亚支,说明蛋白结构可能存在显著差异.转录组数据和RT-qPCR 分析结果表明不同 LeATG 基因在菌丝转色前后的差异表达模式,其中 2 个 LeATG 基因在转色前高表达,5 个在转色后高表达,推测以上 7 个基因可能参与调控菌丝的转色过程.本研究系统鉴定了香菇 LeATG 基因家族,初步揭示了该家族成员在菌丝转色过程中的表达调控模式,为深入解析 LeATG 基因在香菇转色过程中的分子机制奠定了重要基础.
The autophagy-related gene family members in Lentinula edodes(LeATG family members)were identified based on the whole genome data of L.edodes using homologous alignment methods,and their biological information was analyzed.The expression patterns of these family members during mycelial colouring process were analyzed by combining transcriptome sequencing data and RT-qPCR.The results showed that 29 LeATG family members belonging to 23 classes of autophagy-related genes were identified in the L.edodes genome.Chromosomal localization analysis revealed that they were mainly distributed on chromosomes 4 and 1;no distribution was detected on chromosome 9.The full length of LeATG family members ranges from 595 to 6 772 bp.The length of protein sequence encoded by the LeATG gene ranged from 127 to 1 998 aa,with relative molecular weights of 14.8-219.7 kDa and theoretical isoelectric points of 4.27-9.51.Except for LeATG9,LeATG22,and LeATG27,the other 24 LeATG proteins contained no transmembrane domains.The phylogenetic analysis showed that the 29 LeATG proteins clustered into three evolutionary branches,and proteins of the same class were dispersed across different sub-branches,suggesting significant differences in protein structure.Transcriptome data analysis and RT-qPCR revealed differential expression patterns of LeATG genes before and after mycelial colouring process:2 LeATG genes were highly expressed before browning,5 were highly expressed after colouring.It was speculated that these 7 genes might be involved in regulating the mycelial colouring process.This study systematically identified the LeATG gene family in L.edodes,and preliminarily revealed their expression regulation patterns during mycelial colouring process,laying an important foundation for further analysis of the molecular mechanism of the LeATG gene during mycelial colouring process of L.edodes.
胡梦佳;高琪;范阳阳;严冬;王守现;刘宇;宗宪春;刘媛媛
北京市农林科学院植物保护研究所 北京市食用菌工程技术研究中心,北京 100097||牡丹江师范学院生命科学与技术学院,黑龙江 牡丹江 157011北京市农林科学院植物保护研究所 北京市食用菌工程技术研究中心,北京 100097北京市农林科学院植物保护研究所 北京市食用菌工程技术研究中心,北京 100097北京市农林科学院植物保护研究所 北京市食用菌工程技术研究中心,北京 100097北京市农林科学院植物保护研究所 北京市食用菌工程技术研究中心,北京 100097北京市农林科学院植物保护研究所 北京市食用菌工程技术研究中心,北京 100097牡丹江师范学院生命科学与技术学院,黑龙江 牡丹江 157011北京市农林科学院植物保护研究所 北京市食用菌工程技术研究中心,北京 100097
香菇自噬相关基因菌丝转色基因表达
Lentinula edodesautophagy-related genesmycelial colouringgene expression
《菌物学报》 2026 (4)
116-133,18
国家自然科学基金(32202568)北京市农林科学院植物保护研究所改革与发展项目(GGYFZ202512)现代农业产业技术体系北京市食用菌创新团队(BAIC03) This work was supported by the National Natural Science Foundation of China(32202568),the Reform and Development Project,Institute of Plant Protection,Beijing Academy of Agriculture and Forestry Sciences(GGYFZ202512),and the Beijing Innovation Consortium of Agriculture Research System(BAIC03).
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