转抗菌肽基因Ace-AMP-sn抗纹枯病小麦新品系的创制与评价OA

Sharp eyespot has become a major soil-borne fungal disease threatening wheat production in China in re-cent years.Breeding disease-resistant varieties is the most economical and effective strategy for controlling this disease.In this study,the T2 generation wheat variety Yangmai 18 transformed with the onion antimicrobial peptide gene Ace-AMP-sn was used as the material.Through successive generations of resistance identification,three T7 transgenic lines E308,E328,and E332 were obtained.Quantitative real-time PCR(qRT-PCR)results showed that the rela-tive expression levels of Ace-AMP-sn in T7 transgenic lines E308,E328,and E332 were significantly higher than those in the recipient variety Yangmai 18(P＜0.05).Results of disease resistance identification demonstrated that the sharp eyespot disease indices of the three T7 transgenic lines were significantly lower than that of Yangmai 18(P＜0.05),while no significant difference was observed compared with the resistant variety CI12633(P＞0.05).These results indicated that the introduction of Ace-AMP-sn could effectively improve resistance to sharp eyespot in wheat.Furthermore,no signifi-cant differences(P＞0.05)were detected between all T7 transgenic lines and Yangmai 18 in key agronomic traits,including heading date,plant height,spike traits,and yield components,suggesting that the introduction of this gene had no adverse effects on main agronomic traits.This study provides new germplasm with outstanding resistance and excellent agronomic traits for the genetic improvement of sharp eyespot resistance in wheat.