piRNA-33195沉默调控急性髓系白血病CDKN2B基因甲基化的研究OA

Objective To investigate the effect of Piwi-interacting RNA(piRNA)-33195 silen-cing on the methylation status of the cyclin-dependent kinase inhibitor 2B(CDKN2B)gene promoter region in acute myeloid leukemia(AML).Methods Bone marrow samples were collected from 30 patients with iron-deficiency anemia(control group)and 30 patients with AML(disease group),and mononuclear cells were isolated.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression levels of piRNA-33195 and CDKN2B in the two groups,while methyla-tion-specific polymerase chain reaction(MSP)was employed to assess the methylation level of the CDKN2B gene promoter region.AntagomiR-NC(negative control)and antagomiR-piRNA-33195(a specific inhibitor of piRNA-33195)were transfected into bone marrow mononuclear cells from AML patients.After 48 hours,qRT-PCR and MSP were used to detect changes in CDKN2B mRNA expres-sion and promoter methylation levels,respectively.Results The relative expression of piRNA-33195 in bone marrow mononuclear cells from the disease group was higher than that in the control group,while the relative expression of CDKN2B mRNA was lower,with statistically significant differences(P＜0.001).The methylation level of CDKN2B in the disease group was higher than that in the control group,with a statistically significant difference(P＜0.001).After piRNA-33195 silencing,the rela-tive expression level of CDKN2B was higher than that before silencing,while the methylation level of CDKN2B was lower,with statistically significant differences(P＜0.001).Conclusion piRNA-33195 is highly expressed in AML and is closely associated with low CDKN2B expression and hypermethyla-tion.Silencing piRNA-33195 reduces the methylation level of the CDKN2B gene promoter region in bone marrow mononuclear cells from AML patients and upregulates CDKN2B mRNA expression.