2023年北京市淡色库蚊中浓核病毒的鉴定及感染率研究OA
IDENTIFICATION AND INFECTION RATE OF DENSOVIRUS IN CULEX PIPIENS PALLENS IN BEIJING IN 2023
目的 对 2023 年北京市顺义区淡色库蚊样本所携带的病毒进行分子生物学鉴定,并观察不同采集时间淡色库蚊携带病毒的感染率变化情况.方法 采用 CO2 诱蚊灯法采集淡色库蚊,将蚊虫样本分批次研磨开展分子生物学检测分析,对不同采集时间的病毒感染率进行统计学分析.结果 2023 年在北京市顺义区野外采集的淡色库蚊样本中鉴定出 17 株淡色库蚊浓核病毒(Culex pipiens pallens densovirus,CppDNV).病毒基因组编码区核苷酸序列的分析结果显示,CppDNV 为单链 DNA 病毒,该病毒基因组编码区全长为3 335 nt,共编码 2 个非结构蛋白(NS1、NS2)和 1 个衣壳蛋白(VP),3 种蛋白的核苷酸(氨基酸)序列长度分别为 2 376 nt(791 aa)、1 092 nt(363 aa)、1 071 nt(356 aa).经病毒系统进化分析发现,CppDNV 位于 Brevihamaparvovirus 属病毒进化组.经统计学分析,不同采集时间的病毒感染率之间无统计学差异(χ2=4.429,P=0.194).结论 北京市淡色库蚊中鉴定出 CppDNV,且能在当地淡色库蚊自然种群中稳定存在.
Objective This study conducted molecular biological identification of the viruses carried by Culex pipiens pallens specimens collected in Shunyi District,Beijing in 2023,and observed the changes in the infection rate of the viruses carried by Cx.pipiens pallens at different collection times.Methods Cx.pipiens pallens were collected using carbon dioxide mosquito traps.The mosquito samples were ground in batches and analyzed by molecular biology technologies.The virus infection rate at different collection times was analyzed statistically.Results 17 strains of Culex pipiens pallens densovirus(CppDNV)were identified from Cx.pipiens pallens samples collected in Shunyi District,Beijing,in 2023.The nucleotide sequence analysis of the virus genome coding region showed that CppDNV was a single-stranded DNA virus with a total length of 3 335 nt,encoding 2 non-structural proteins(NS1,NS2)and 1 capsid protein(VP).The nucleotide(amino acid)sequence lengths of the three proteins were 2 376 nt(791 aa),1 092 nt(363 aa)and 1 071 nt(356 aa),respectively.Phylogenetic analysis showed that CppDNV was located in genus Brevihamaparvovirus.Statistical analysis showed no significant difference in infection rates across collection times(χ2=4.429,P=0.194).Conclusions CppDNV was identified in Cx.pipiens pallens in Beijing,and it was stably maintained in this natural population.
许秀燕;佟颖;张勇;阎婷;朱思捷;李静;刘美德;张洪江;刘婷;李秋红;周小洁
北京市疾病预防控制中心消毒与有害生物防制所,北京 100013北京市疾病预防控制中心消毒与有害生物防制所,北京 100013首都医科大学公共卫生学院,北京 100069||北京市疾病预防控制中心消毒与有害生物防制所,北京 100013北京市疾病预防控制中心消毒与有害生物防制所,北京 100013||首都医科大学公共卫生学院,北京 100069北京市顺义区疾病预防控制中心消毒科,北京 101300北京市疾病预防控制中心消毒与有害生物防制所,北京 100013北京市疾病预防控制中心消毒与有害生物防制所,北京 100013北京市疾病预防控制中心消毒与有害生物防制所,北京 100013北京市疾病预防控制中心消毒与有害生物防制所,北京 100013北京市疾病预防控制中心消毒与有害生物防制所,北京 100013北京市疾病预防控制中心消毒与有害生物防制所,北京 100013
医药卫生
细小病毒Brevihamaparvovirus属淡色库蚊浓核病毒感染率
ParvovirusBrevihamaparvovirusCulex pipiens pallensDensovirusInfection rate
《寄生虫与医学昆虫学报》 2026 (1)
25-30,6
基于机器学习和气象因素的北京市蚊虫及蚊媒传染病监测预警研究(首发2024-2G-30116)
评论