实炎方对溃疡性结肠炎小鼠肠道菌群及Th17细胞的调节影响OA

Objective To observe the effects of Shiyan Decoction on intestinal microbiota and Th17 cells in mice with ulcerative colitis(UC),and to explore its mechanism in the treatment of UC.Methods A total of 48 mice were randomly divided into a blank group,a model group,a mesalazine group,a low-dose Shiyan Decoction group,a medium dose Shiyan Decoction group,and a high-dose Shiyan Decoction group,with 8 mice in each group.The model group was given 3%DSS solution for free diet modeling,while the low-dose Shiyan Decoction group,medium dose Shiyan Decoction group,high-dose Shiyan Decoction group,and mesalazine group were given 3%DSS solution for free drinking for 7 days,and then switched to sterile water for 7 days.During the entire process,the mice were given 15g/kg Shiyan Decoction,30g/kg Shiyan Decoction,60g/kg Shiyan Decoction,or 0.5g/kg mesalazine by gavage.The DAI scores,colon length were recorded,and HE and MASS staining were used to observe the effects of Shiyan Decoction on intestinal mucosal inflammation in UC mice and score them.16S rRNA sequencing was used to analyze the changes in gut microbiota of mice treated with Shiyan Decoction,and flow cytometry was used to determine the proportion of Th17 cells in mouse serum.Results Compared with the model group,after the intervention with Shiyan Decoction,the DAI scores of mice were significantly reduced,and the colon length was longer than that of the model group and tended to be normal,and the HE scores and MASSON staining scores were also significantly lower than those of the model group.After the intervention with Shiyan Decoction,there exhibited significant changes in the gut microbiota structure of mice,mainly characterized by the phylum Bacteroidetes,order Lactobacillales,genus Lactobacillaceae,family Lactobacillaceae,and phylum Firmicutes.After the intervention with Shiyan Decoction,the content of Th17 cells in mice was significantly lower than that in the model group.Conclusion Shiyan Decoction can improve intestinal immune balance and alleviate intestinal inflammation by regulating the structure of intestinal microbiota,increasing the content of beneficial bacteria such as lactobacilli,and reducing the activation and activity of Th17 cells.