玉米ZmTCP14基因的筛选、生物信息学分析及植物表达载体的构建OA

TCP is a family of transcription factors unique to plants containing bHLH structures,in-volved in the regulation of development and abiotic stress in plant cells.In this study,a significantly down-regulated target gene ZmTCP14 was screened from TCP transcription factor family by fluores-cence quantitative expression analysis under drought stress.Combined with bioinformatics,the bio-logical structure and function prediction analysis of ZmTCP14 gene were carried out,and finally,the ZmTCP14 gene was cloned and a plant overexpression vector was constructed.The results showed that ZmTCP14 was highly correlated with sorghum(Sorghum bicolor),rice(Oryza sativa)and millet(Setaria italica).It contained 20 motifs with highly conserved domains,and the homologous protein alignment was consistent with the phylogenetic tree analysis results.This protein was a class of hy-drophilic proteins located in the nucleus,and contained multiple phosphorylation sites,but no lipo-protein signal peptides and transmembrane structures.Analysis of the promoter element sequences revealed a close relationship between ZmTCP14 gene and stress and light response.Finally,a single target band of 2 267 bp was successfully cloned,and the plant overexpression vector pCAMBIA3301-ZmTCP14-Bar was constructed.This study lays a foundation for the subsequent exploration of the regulatory mechanism of plant drought-related genes.