不同方法对貉毛囊基因组DNA提取效果比较OA

Genomic DNA is one of the essential materials for molecular breeding,and using hair follicles to extract genomic DNA is a simple and efficient method.To compare the effects of different extraction methods,the number of raccoon dog hair follicles and sampling sites on the efficiency of genomic DNA extraction,with the aim of determining the optimal protocol for extracting genomic DNA from raccoon dog hair follicles,40 healthy raccoon dogs of different ages and genders were selected.Hair follicle samples were collected from the mid-dorsal region and tail of each individual.DNA was extracted using the centrifugal column method and the magnetic bead method,and the efficiency of genomic DNA extraction was compared based on the number of hair follicles and sampling sites.The results showed that the centrifugal column method outperformed the magnetic bead method,and extracting 100 or more hair follicles could meet the requirements for subsequent experiments.Additionally,the sampling site had no significant impact on DNA extraction quality.The findings provide a scientific approach for obtaining genomic materials from fur-bearing animals such as raccoon dog and lay a foundation for genetic breeding research.