乌苏里貉PRLR和RBP4基因多态性检测及其与繁殖性能的关联分析OA

To investigate the relationship between different genotypes and the total litter size of Ussuri raccoon dogs,the PCR-SSCP method was employed to analyze the polymorphisms of the retinol-binding protein 4(RBP4)gene and the prolactin receptor(PRLR)gene in 38 Ussuri raccoon dogs.The results showed that one polymorphic site(g.42521676 C>G)was identified in the RBP4 gene and one(g.150456010 G>A)in the PRLR gene,both of which were in a state of Hardy-Weinberg disequilibrium.The dominant genotype for both the RBP4 and PRLR genes in the Ussuri raccoon dog population was the AA genotype,with the A allele being the dominant allele.For the RBP4 gene polymorphic site,the frequencies of the AA,BB,and AB genotypes were 0.500,0.184,and 0.316,respectively,while the frequencies of the A and B alleles were 0.658 and 0.342,respectively.Significant differences in total litter size were observed among different genotypes,with the AA genotype having 4.6 more offspring than the BB genotype.For the PRLR gene polymorphic site,the frequencies of the AA and AB genotypes were 0.711 and 0.289,respectively,and the frequencies of the A and B alleles were 0.856 and 0.144,respectively.The AA genotype had 3.6 more offspring than the AB genotype.The results provide a theoretical basis for the screening of molecular markers for early breeding selection in this species.