长江口葛氏长臂虾肠道菌群结构及其潜在功能的研究OA

To gain insights into the gut microbial community structure and its potential functions of Palaemon graviera,high-throughput sequencing technology was employed to analyze samples collected from the Yangtze River Estuary(31°06'00″N to 31°24'00″N,122°00'00″E to 122°14'30″E)in August 2022.Further,Kyoto Encyclopedia of Genes and Genomes predictions were performed to elucidate the functional roles of the gut microbiota.The results revealed that the number of high-quality sequencing reads ranged from 33 054 to 37 110 for Palaemon graviera from the Tangtze Estuary.Through clustering analysis,a total of 367 operational taxonomic units were identified.The gut microbial communities of Palaemon graviera were dominated by Proteobacteria and Firmicutes.And Psychrobacter,Macrococcus,Mycobacterium,and Bradyrhizobium emerged as the dominant genera.According to the Kyoto Encyclopedia of Genes and Genomes pathway analysis,the most enriched pathways at various levels were metabolism,with pyrimidine metabolism,propionate metabolism,amino acid metabolism,and purine metabolism being the primary ones.In addition,the intestinal flora of Palaemon graviera also possessed functions related to cellular processes,environmental information processing,genetic information processing,and disease.According to the gut microbiota structural analysis and Kyoto Encyclopedia of Genes and Genomes based functional predictions,it is tentatively inferred that the Palaemon graviera in the Yangtze River Estuary feeds on diets containing both animal and plant proteins,carbohydrates,as well as some organic detritus.This study lays a foundation for future research on healthy aquaculture,environmental ecology,and wild breeding of Palaemon graviera in the Yangtze River Estuary.Additionally,it contributes to the literature on the application and development of high-throughput sequencing technologies.