大口黑鲈脂肪酸合成酶FASN基因生物信息学分析OA

Fatty acid synthase(FASN)is a key rate-limiting enzyme for de novo fatty acid synthesis in cells,and its activity directly affects the synthesis of fatty acids in animals.In this article,the FASN gene and amino acid se-quence of largemouth bass were partially analyzed using bioinformatics methods.Alignment was performed using DNAMAN software,and the nucleotide similarity alignment value obtained through operation was 83.02%,and the protein similarity alignment value was 90.94%.The construction results of the gene evolution tree among multiple species showed that the genetic relationship between largemouth bass and the other 18 species was different,but it was most closely related to smallmouth bass.ClustalW alignment was performed using Megalign software,and the homology obtained through the sequence distances operation was 93.7%.Based on the analysis of the physicochemi-cal properties of the fatty acid synthase protein,it was found that the contents of isoleucine,alanine,glycine,and valine in the protein amino acids of largemouth bass were significantly higher than those of other amino acids;the instability coefficient value ranged from 38.90 to 46.44,which was a stable protein;the isoelectric point of the pro-tein ranged from 5.70 to 6.13,which was an acidic protein.Combining the above content,this article analyzed and studied the FASN gene using various analysis methods and software tools,which is helpful for the continued study of the activity mechanism of FASN in fatty acid synthesis in species.