贵州不同产区杜仲内生菌与根际微生物群落多样性及功能分析OA

In order to reveal the intrinsic relationship between endophytic and rhizosphere soil microbial communities in the bark and fibrous roots of E.ucommia ulmoides and their environmental adaptability,High-throughput sequencing was performed on endophytic microorganisms from bark and roots,as well as rhizosphere soil microorganisms of E.ulmoides collected from Daozhen,Weng'an,and Bijie production areas in Guizhou in this study.Bioinformatics methods were employed to analyze the microbial structure,species correlations,and functional predictions.The results showed that,NMDS analysis showed that bacterial communities clustered specifically by tissue compartment(bark,root,soil),while fungal communities were influenced by both production area and tissue compartment.The Bijie production area had the lowest number of endophytic ASVs but the highest number in rhizosphere soil.It also exhibited the highest abundance of potentially pathogenic bacterial phenotypes and the lowest stress tolerance.Dominant bacterial phyla included Pseudomonadota and Actinomycetota,with the abundance of Pseudomonadota in bark and rhizosphere soil showing a trend of DZ＜WA＜BJ,while Actinomycetota showed the opposite pattern.Dominant bacterial genera,such as Sphingomonas,Bradyrhizobium,and Actino phytocola,exhibited tissue specificity.Among fungi,the phylum Glomeromycota reached an abundance of 9.63％in Daozhen roots,and the genus Mortierella was dominant in the rhizosphere soil of Daozhen and Weng'an.Network analysis indicated that microbial corre-lations were tissue-specific,with endophytic and rhizosphere microbes mostly showing negative correlations.Functional prediction revealed differences in microbial phenotypes and trophic types among production areas,with Bijie showing higher potential pathogenicity,and arbuscular mycorrhizal fungi were enriched in Daozhen roots.In conclusion,the microbial community structure of E.ulmoides exhibited clear tissue-specific and pro-duction area-specific characteristics.Community assembly was co-regulated by the micro-environment and pro-duction area conditions.