山杨PdNAC2基因序列特征及表达分析OA

In order to analyze the expression pattern of PdNAC2 in Populus davidiana and obtain candidate genes for drought-resistant strains,the PdNAC2 gene,which had been previously screened from the drought transcriptome of P.davidiana,was selected as the research object in this study.The cDNA isolated from P.davidiana leaves treated with drought stress was used as the template to amplify the target fragment of the PdNAC2 gene via PCR.Bioinformatics analysis was performed on the PdNAC2 gene to characterize its sequence features,and the expression pattern of PdNAC2 was investigated using qRT-PCR.The results showed that the open reading frame(ORF)of the PdNAC2 gene was 852 bp in length,and it was predicted to encode 284 amino acids.Multiple sequence alignment revealed that the conserved domain sequence of PdNAC2 shared extremely high sequence identity with NAC2 proteins from other species.Based on the differences in conserved structures among different gene families,PdNAC2 from P.davidiana was found to be closely related to that of Arabidopsis thaliana.Analysis of the expression pattern via qRT-PCR indicated that the relative expression level of the PdNAC2 gene fluctuated with the duration of stress.On the 8th day of stress,the relative expression level of the PdNAC2 gene exhibited an initial upward trend followed by a downward trend.Collectively,the expression of the PdNAC2 gene could respond to drought treatment,and this gene might be associated with drought resistance in P.davidiana.