树莓Ri3GT基因克隆及表达模式分析OA

To investigate the sequence characteristics,expression pattern,and promoter cis-elements of the raspberry flavonoid 3-O-glucosyltransferase gene(Ri3GT),postharvest raspberry fruits were used as experimental materials.The Ri3GT gene and its promoter sequence were cloned by PCR and subjected to bioinformatic anaylsis,and the expression pattern of Ri3GT was detected by quantitative real-time PCR.The results showed that the full-length cDNA of Ri3GT was 1 395 bp,encoding 464 amino acids,with multiple phosphorylation sites and no signal peptide.The protein belonged to the UDP glucosyltransferase family and contained a PSPG domain.It had the closest genetic relationship with strawberry 3GT,and bound to cyanidin mainly through five types of interactions,including conventional hydrogen bonds and Pi-Cation interactions.The expression level of Ri3GT increased significantly on day 2 of postharvest storage,and this trend was positively correlated with anthocyanin content.Light and methyl jasmonate response elements were identified in the Ri3GT promoter.These results provide a reference for elucidating the role of the Ri3GT gene in anthocyanin biosynthesis in postharvest raspberry fruits.