基于网络药理学和分子对接分析黄芪缓解奶牛乳房炎作用机制OA

To explore the mechanism of Astragalus membranaceus in alleviating bovine mastitis,network pharmacology methods were employed,supplemented by molecular docking simulation validation,aiming to provide a reference for the clinical application of Astragalus membranaceus.The active ingredients of Astragalus membranaceus and targets related to bovine mastitis were screened and identified using the traditional Chinese medicine systems pharmacology(TCMSP)database,gene cards and the online mendelian inheritance in man(OMIM)database.Data were standardized using the UniProt databases.The venny 2.1 online tool was utilized to identify common targets between Astragalus membranaceus and bovine mastitis.Molecular docking simulations were performed using AutoDockTools 1.5.6 software to explore intermolecular interactions and potential mechanisms.The results showed that 16 main active components of Astragalus membranaceus(including quercetin,kaempferol,etc)were screened,along with 377 bovine mastitis-related targets,yielding 21 common targets.Key targets such as TNF and IL1B were identified through PPI network analysis.Good binding activity between the active components of Astragalus membranaceus and some key targets was confirmed by molecular docking validation.A total of 61 biological processes,8 molecular functions,and 9 cellular components were obtained from GO functional enrichment analysis,and 81 signaling pathways were identified via KEGG pathway enrichment analysis.Finally,it was concluded that 16 main active ingredients in Astragalus membranaceus,such as quercetin and kaempferol,might exert therapeutic effects by acting on key targets like TNF,IL6,IL1B,and alleviate bovine mastitis through 81 pathways,including the malaria pathway and the IL-17 signaling pathway.