脂必泰调控PPARγ/CD36通路抗动脉粥样硬化的实验研究OA

Objective:To investigate the mechanism by which Zhibitai Capsule regulates atherosclerosis in mice based on the peroxisome proliferator-activated receptor γ(PPARγ)/cluster of differentiation 36(CD36)pathway.Methods:Thirty-six male APOE-/-mice were randomly divided into the control group(Control),model group(Model),atorvastatin group(Atorvastatin,3 mg·kg-1),Zhibitai low-dose group(ZBT-L,31.2 mg·kg-1),Zhibitai Capsule medium-dose group(ZBT-M,62.4 mg·kg-1),and Zhibitai high-dose group(ZBT-H,124.8 mg·kg-1),with 6 mice in each group.Except for the Control group,the remaining mice were fed a high-fat diet to establish the atherosclerosis model and were administered orally once daily for 12 consecutive weeks.Hematoxylin-e-osin(HE)staining and Oil Red O staining were used to observe aortic pathological morphology and lipid accumulation in mice;the col-orimetric method was used to determine serum levels of total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C);ELISA was employed to detect serum oxidized low-density lipopro-tein(ox-LDL)levels;and Western blot was used to assess aortic PPARγ and CD36 protein expression levels.Results:Oil Red O stai-ning showed that the aorta of mice in the Control group was smooth,and no obvious red-stained lipid droplets were observed in the liv-er;in the Model group,obvious red-stained lipids were seen in the aortic intima,and more red-stained lipid droplets were observed in hepatocytes.HE staining showed that the structure of each layer of the aorta in the Control group was normal;in the Model group,the medial layer of the aorta was disordered,with obvious plaques visible;in the Atorvastatin group,the aortic structure was disordered,but no plaque formation was observed;in the ZBT-H group,the aortic structure was normal,with smooth vessel walls.Compared with the Control group,the Model group had increased aortic lipid content and higher percentages of lesions in the aorta and liver(P<0.01);compared with the Model group,the Atorvastatin group,ZBT-M group,and ZBT-H group had decreased aortic lipid content and lower percentages of lesions in the aorta and liver(P<0.05).Compared with the Control group,the Model group had increased serum levels of TC,TG,LDL-C,and ox-LDL,and decreased HDL-C levels(P<0.01);compared with the Model group,the Atorvastatin group,ZBT-M group,and ZBT-H group had decreased serum levels of TC,TG,LDL-C,and ox-LDL,and increased HDL-C levels(P<0.05).Compared with the Control group,the Model group had decreased aortic PPARγ protein expression and increased CD36 protein expression(P<0.01);compared with the Model group,the Atorvastatin group,ZBT-M group,and ZBT-H group had increased aor-tic PPARγ protein expression and decreased CD36 protein expression(P<0.01).Conclusion:Zhibitai Capsule can prevent atheroscle-rotic lesions,and its mechanism may be related to the regulation of the PPARγ/CD36 signaling pathway and the improvement of macro-phage lipid metabolism.