贝莱斯芽孢杆菌MD4抑制番茄灰霉病的生物防治潜力OA

[Objective]To investigate the biocontrol potential of strain MD4 against tomato gray mold and its effect on fruit quality,and to provide theoretical support for the development and applica-tion of tomato gray mold microbial agents.[Method]The inhibitory effect of strain MD4 against Botrytis cinerea was determined by plate confrontation culture.The strain was identified through morphological,physiological and biochemical characteristics,and 16S rDNA sequence analysis.The control efficacy against tomato gray mold and the effects on the quality of postharvest tomato fruits were evaluated.[Result]1)Strain MD4 exhibited an inhibition rate of 70.40%against B.cinerea and was identified as Bacillus velezensis.2)MD4 treatment induced morphological abnor-malities in B.cinerea,including excessive hyphal branching,swelling,and cytoplasmic leakage.3)Inoculation with strain MD4 effectively reduced the incidence and disease index of tomato gray mold,with control efficacies of 65.31%and 55.21%for leaf and fruit infections,respectively.4)Strain MD4 could increase the contents of vitamin C,soluble solids,total phenols,and flavo-noids in tomato fruits,and delay the decrease in fruit weight loss rate caused by B.cinerea infec-tion.[Conclusion]Strain MD4 is a promising biocontrol strain with potential for application in tomato gray mold management.