毛白杨RBOH基因家族鉴定与表达分析OA

[Objective]This study was conducted to identify members of the RBOH(respiratory burst oxidase homologue)family in Populus tomentosa and investigate their functions and roles in the growth and development of this species,thereby providing an informational foundation for stress resistance improvement in Populus tomentosa.[Method]The whole genome data of Populus tomentosa were sys-tematically analyzed,19 members of the RBOH family were identified,and their evolutionary relation-ships,gene structures,conserved motifs and domains,promoter cis-acting elements,and spatiotempo-ral expression levels were studied.[Results]The RBOH family members of Populus tomentosa are divided into 5 subfamilies,distributed on 11 chromosomes.Evolutionary analysis shows that RBOH members of Populus tomentosa have high homology with those of Arabidopsis and rice.The analysis of cis-acting elements shows that the promoter region of PtoRbohs in Populus tomentosa contains multiple elements that respond to light,hormones,and stress signals,indicating their possible involvement in various biological processes.Temporal and spatial expression analysis showed that PtoRboh4A was highly expressed in the roots of Populus tomentosa,while PtoRboh11D expression gradually increased with the degree of stem lignification.PtoRboh2D and PtoRboh3D were preferentially expressed in mature leaves.In the stem,PtoRboh4A was preferentially expressed in the phloem;PtoRboh1A,PtoR-boh1D and PtoRboh5A were preferentially expressed in the xylem.[Conclusion]The characteristics and expression patterns of the RBOH family members in Populus tomentosa were systematically ana-lyzed.The sequence and evolution of the RBOH family members in Populus tomentosa were relatively conservative,and they exhibited functional diversity.The high expression patterns of genes such as PtoRboh2D,PtoRboh3D,PtoRboh4A,and PtoRboh11D suggest that they may have important biologi-cal functions.